Regulatory

Part:BBa_K4194000

Designed by: Jiong Huang   Group: iGEM22_BUCT-China   (2022-09-23)


A promoter that is inhibited by LacI.

Lac operator is added downstream of the promoter p(Bla) from E. coli transposon Tn3. Promoter p(Bla) was originally a medium-strength constitutive promoter, but the downstream lac operator could bind to LacI protein to repress promoter p(Bla). IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None